Journal: Virology

Article Title: The low pH-dependent entry of avian reovirus is accompanied by two specific cleavages of the major outer capsid protein mu 2C.

doi: 10.1006/viro.1996.0235

Figure Lengend Snippet: FIG. 8. Entry-related cleavages of m2C are cell type- and virus strain-independent. (A) Radiolabeled, purified avian reovirus strain 176 (176) and mammalian reovirus serotype 3 Dearing (T3) were used in an internalization assay, as described in the legend of Fig. 3, using QT6 cells or L929 cells, respectively. Samples were harvested at t Å 0, 45, or 90 min after virus adsorption and analyzed by SDS–PAGE and autoradiography. The locations of individual viral proteins are indicated on the sides of the figure. (B) Radiolabeled, purified avian reovirus strain S1133 was used in an internalization assay, as described in the legend of Fig. 3, using QT6 cells (QT6) or Vero cells (VERO). Samples were harvested at t Å 15, 30, 60, or 90 min after virus adsorption and analyzed by SDS–PAGE and autoradiography. The locations of individual viral proteins are indicated on the sides of the figure.

Article Snippet: The mammalian reovirus serotype 3 (Dearing strain)Since enveloped viruses utilize the same mechanism was obtained from Patrick Lee (University of Calgary)to mediate the membrane interactions involved in both and was plaque purified and grown in murine L929 cells.virus entry and syncytium formation (Bentz, 1993), avian Murine L929 cells (ATCC number 1-CCL) and Vero cellsreovirus entry was investigated to determine whether the (ATCC number 81-CCL) were obtained from the Ameri-neutral pH, fusion-inducing activity of s3 might bypass can Type Culture Collection.

Techniques: Virus, Purification, Adsorption, SDS Page, Autoradiography

Journal: PLoS ONE

Article Title: Attenuation of Vaccinia Tian Tan Strain by Removal of Viral TC7L-TK2L and TA35R Genes

doi: 10.1371/journal.pone.0031979

Figure Lengend Snippet: Effects of MVTT1, MVTT2, MVTT3, or VTT (0.05 PFU/cell) and infection times on the cell viabilities of HeLa (A), MDCK (B), PK(15) (C), BHK-21 (D), and Vero (E). Cells were seeded in 96-well plates (1×10 4 cells/well) one day before they were infected with 0.05 PFU/cell of MVTT1, MVTT2, MVTT3, or VTT. Cell viability was measured daily for 4 d by MTT colorimetric assay. All measurements were performed in triplicate. Data are presented as mean ± SD.

Article Snippet: BHK-21 hamster kidney cells, PK(15) porcine kidney cells, HeLa human cervical adenocarcinoma epithelial cells, Madin-Darby canine kidney (MDCK) epithelial cells, and Vero African green monkey kidney cells were obtained from the China Center for Type Culture Collection.

Techniques: Infection, Colorimetric Assay

Journal: PLoS ONE

Article Title: Attenuation of Vaccinia Tian Tan Strain by Removal of Viral TC7L-TK2L and TA35R Genes

doi: 10.1371/journal.pone.0031979

Figure Lengend Snippet: Confluent monolayers of PK(15), MDCK, HeLa, BHK-21 and Vero cells in 12-well plates were infected with 0.05 PFU/cell of MVTT1, MVTT2, MVTT3, or VTT viruses. The plates were incubated at 37°C for 2 d prior to staining with 0.1% crystal violet. The pathogenicity of MVTT1, MVTT2, and MVTT3 apparently decreased in all five cell lines (compared with VTT as the control).

Article Snippet: BHK-21 hamster kidney cells, PK(15) porcine kidney cells, HeLa human cervical adenocarcinoma epithelial cells, Madin-Darby canine kidney (MDCK) epithelial cells, and Vero African green monkey kidney cells were obtained from the China Center for Type Culture Collection.

Techniques: Infection, Incubation, Staining, Control

Journal: PLoS ONE

Article Title: Attenuation of Vaccinia Tian Tan Strain by Removal of Viral TC7L-TK2L and TA35R Genes

doi: 10.1371/journal.pone.0031979

Figure Lengend Snippet: PK(15), MDCK, HeLa, BHK-21 and Vero cells infected with 0.05 MOI of VTT and the mutants, and then the viruses were harvested and titrated in BHK-21 cells. Virus titer was determined by measuring the plaque assays.

Article Snippet: BHK-21 hamster kidney cells, PK(15) porcine kidney cells, HeLa human cervical adenocarcinoma epithelial cells, Madin-Darby canine kidney (MDCK) epithelial cells, and Vero African green monkey kidney cells were obtained from the China Center for Type Culture Collection.

Techniques: Infection, Virus